Journal: Virology

Article Title: Human cytomegalovirus-infected cells release extracellular vesicles that carry viral surface proteins

doi: 10.1016/j.virol.2018.08.008

Figure Lengend Snippet: (A) stain-free blot representing the spectrum of proteins obtained for HCMV and EV fractions after separation of AD169 HCMV pre-paration using a iodixanol step-gradient centrifugation. (B) Detection of EV-associated proteins CD63 (~60 kDa), Rab27A (~30 kDa), and calnexin (~25 kDa) proteins in both fractions. (C) Detection of HCMV capsid protein MCP (~150 kDa) and HCMV tegument protein, pp150 (~160 kDa) in HCMV and EV fractions after separation of AD169 viral preparation using a iodixanol step-gradient centrifugation.

Article Snippet: 10 μg of proteins were loaded on a 4–20% precast polyacrylamide gel (Bio-Rad Laboratories, Hercules, CA) and separated by SDS-PAGE, then transferred to PVDF membranes and probed with anti-CD63 (1 μg/ml, Thermo Fisher Scientific, Waltham, MA), anti-Calnexin (1 μg/ml, Thermo Fisher Scientific, Waltham, MA), anti-Rab27A (1 μg/ml, Thermo Fisher Scientific, Waltham, MA), anti-HCMV capsid MCP (2 μg/ml), and anti-HCMV tegument pp150 (2 μg/ml) monoclonal primary anti-mouse monoclonal antibodies (clones 28–4 and 36–14) and then goat peroxidase-conjugated anti-mouse IgG secondary anti-body (Bio-Rad Laboratories, Hercules, CA).

Techniques: Staining, Gradient Centrifugation

Journal: Cell Reports

Article Title: Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity

doi: 10.1016/j.celrep.2023.113634

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-β-catenin (Clone 14) , BD Biosciences , Cat# 610153; RRID: AB_397554.

Techniques: Recombinant, Virus, Sequencing, Modification, Multiplex Assay, Positive Control, Negative Control, Mass Spectrometry, Knock-In, Knock-Out, Transgenic Assay, Software

Journal: Journal of Mammary Gland Biology and Neoplasia

Article Title: SFRP1 Expression is Inversely Associated With Metastasis Formation in Canine Mammary Tumours

doi: 10.1007/s10911-023-09543-z

Figure Lengend Snippet: β-catenin protein expression (IHC) in CMTs with and without metastasis. A Immunohistochemistry for β-catenin in the same six examples of CMTs with (M1-6) and without (N1-6) metastasis as shown in Fig. . Membrane staining did correlate with metastasis status, but not with SFRP1 expression. Bars represent 200 µm

Article Snippet: Primary antibodies rabbit anti-SFRP1 mAb (ab126613; Abcam; Berlin, Germany) and mouse anti-β-catenin (clone 14; 610,154; BD Biosciences, Heidelberg, Germany) were applied at a dilution of 1:200 in PBS/BSA 2% and incubated overnight at 4 °C.

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Journal of Mammary Gland Biology and Neoplasia

Article Title: SFRP1 Expression is Inversely Associated With Metastasis Formation in Canine Mammary Tumours

doi: 10.1007/s10911-023-09543-z

Figure Lengend Snippet: Immunohistochemistry results for SFRP1 as well as membrane (m) or nuclear (n) associated β-catenin in the new cohort of 33 CMTs compared to Sfrp1 mRNA expression (0–3), grade (1–3), and metastasis status (neg, pos)

Article Snippet: Primary antibodies rabbit anti-SFRP1 mAb (ab126613; Abcam; Berlin, Germany) and mouse anti-β-catenin (clone 14; 610,154; BD Biosciences, Heidelberg, Germany) were applied at a dilution of 1:200 in PBS/BSA 2% and incubated overnight at 4 °C.

Techniques: Immunohistochemistry, Expressing